A Fluorometric CYP19A1 (Aromatase) Activity Assay in Live Cells.
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Abstract | :
Inhibition of estrogen synthesis is an integral component of the frontline pharmacologic therapy for the treatment of estrogen receptor positive cancers. However, there is currently no direct, high-throughput-ready assay for aromatase (also known as CYP19A1) that can be performed in live cells. Herein we present a cell-based assay that allows for multiplexed assessment of enzyme activity, protein half-life, cell viability, and identification of inhibitors with slow off-rates. |
Year of Publication | :
2021
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Journal | :
ChemMedChem
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Volume | :
16
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Issue | :
18
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Number of Pages | :
2845-2850
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Date Published | :
2021
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ISSN Number | :
1860-7179
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URL | :
https://doi.org/10.1002/cmdc.202100326
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DOI | :
10.1002/cmdc.202100326
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Short Title | :
ChemMedChem
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