Utilizing the Modified T-Maze to Assess Functional Memory Outcomes After Cardiac Arrest.
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Abstract | :
Evaluating mild to moderate cognitive impairment in a global cerebral ischemia (i.e. cardiac arrest) model can be difficult due to poor locomotion after surgery. For example, rats who undergo surgical procedures and are subjected to the Morris water maze may not be able to swim, thus voiding the experiment. New Method: We established a modified behavioral spontaneous alternation T-maze test. The major advantage of the modified T-maze protocol is its relatively simple design that is powerful enough to assess functional learning/memory after ischemia. Additionally, the data analysis is simple and straightforward. We used the T-maze to determine the rats' learning/memory deficits both in the presence or absence of mild to moderate (6 min) asphyxial cardiac arrest (ACA). Rats have a natural tendency for exploration and will explore the alternate arms in the T-maze, whereas hippocampal-lesioned rats tend to adopt a side-preference resulting in decreased spontaneous alternation ratios, revealing the hippocampal-related functional learning/memory in the presence or absence of ACA. |
Year of Publication | :
2018
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Journal | :
Journal of visualized experiments : JoVE
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Issue | :
131
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Date Published | :
2018
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URL | :
https://dx.doi.org/10.3791/56694
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DOI | :
10.3791/56694
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Short Title | :
J Vis Exp
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